On May 12, 2025, Tianjin Zhonghe Gene Technology Co., Ltd. (hereinafter referred to as Zhonghe Gene) and Tianjin Tianbao Holding Co., Ltd. signed a DNA synthesis project. This marks that the new generation of DNA synthesis technology - enzymatic DNA biosynthesis technology has officially stepped into industrialization, injecting new momentum of "Intelligent Manufacturing in China" into the field of synthetic biology.

It is understood that DNA synthesis technology is a key common underlying technology and core enabling technology in synthetic biology. Its synthesis principles are mainly divided into chemical methods and biological methods, and the existing commercialized DNA synthesis technologies and equipment are mainly based on chemical methods. At present, the synthesis length and cost of chemical DNA synthesis methods have reached their limits. However, this technology still has problems such as low single-step accuracy, cumbersome steps for long-fragment DNA synthesis, high synthesis cost, and environmental pollution, which restrict the rapid development of the synthetic biological manufacturing industry. Therefore, DNA biosynthesis method has become a new generation of DNA synthesis technology, which shows advantages over traditional chemical synthesis methods in terms of length, accuracy, cost, and environmental protection.

Based on this, Zhonghe Gene has independently developed the TIESyno-96 DNA biosynthesis instrument, which can realize de novo synthesis of DNA molecules through aqueous phase bioenzymatic reactions without relying on DNA templates. Equipped with supporting kits, this product can efficiently, accurately and controllably synthesize single-stranded DNA, without the need for harsh experimental environments. The reagents used are environmentally friendly, and the synthesized DNA has stable and reliable quality. In addition, when used in conjunction with a gene splicer, this equipment can form Kb-level gene synthesis capabilities based on biological methods, which is very suitable for building Kb-level biological gene synthesis platforms in conventional laboratories. It has broad application prospects in many fields such as synthetic biology, biomedicine, basic scientific research, and DNA information storage.